Substrati elisa ELISA

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ELETTROFORESI

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REBlot Chemicon

RELIABlot Bethyl

Rilevazione His-Proteine

APS Temed compresse

Agarosio

TMB SureBlue

Substrati ELISA

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Substrate	pNPP	BluePhos	ABTS	SureBlue/TMB	SureBlue Reserve^TM	LumiGLO
Enzyme	AP	AP	HRP	HRP	HRP	HRP
Type	Chromogenic	Chromogenic	Chromogenic	Chromogenic	Chromogenic	Chemiluminescent
Kit Components	2 (Tablet+Buffer)	2	1 or 2	1 or 2	1	2
Detection Limit	10^-13moles of AP	10^-13moles of AP	10^-13moles of HRP	10^-15moles of HRP	5 x 10^-16moles of HRP	10^-18moles of HRP
Kinetics	Fast	Fast	Slow	Fast	Fast	Fast-Max light output in 5 minutes
Stability of Working Solution	24 hours	1 hour	1-C Min 1 year 2-C 24 hours	1-C Min 1 year 2-C 24 hours	2 years from the date of manufacture	24 hours
Hazards	None	None	None	1-C None 2-C Contains DMF	None	None
Color Before Stopping	Yellow	Blue	Blue-Green	Blue	Blue	NA
Absorbance Before Stopping	405-410 nm	595-650 nm	405-410 nm	650 nm	650 nm	425 nm (emmission)
Stop Reagent	5% EDTA	2.5% EDTA	ABTS Stop Solution	TMB Stop Solution	TMB Stop Solution	NA
Color After Stopping	Yellow	Blue	Blue-Green	Yellow	Yellow	NA
Absorbance After Stopping	405-410 nm	595-650 nm	405-410 nm	450 nm	450 nm	425 nm
Amplification After Stopping	None	None	None	2-3 fold	2-3 fold	NA

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