{"id":6656,"date":"2024-11-15T12:57:28","date_gmt":"2024-11-15T12:57:28","guid":{"rendered":"https:\/\/staging.prodottigianni.com\/flow-cytometry\/"},"modified":"2026-05-07T08:29:42","modified_gmt":"2026-05-07T08:29:42","slug":"flow-cytometry","status":"publish","type":"page","link":"https:\/\/www.prodottigianni.com\/en\/life-science-services\/flow-cytometry\/","title":{"rendered":"Flow Cytometry"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"6656\" class=\"elementor elementor-6656 elementor-948\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-e49ce35 e-con-full e-flex e-con e-parent\" data-id=\"e49ce35\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t<div class=\"elementor-element elementor-element-8961afc e-con-full e-flex e-con e-child\" data-id=\"8961afc\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-f96f272 elementor-widget elementor-widget-heading\" data-id=\"f96f272\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">Flow Cytometry Line<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4ee1707 elementor-widget elementor-widget-heading\" data-id=\"4ee1707\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">A broad and complete line for flow cytometric analysis.<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-e66482a e-flex e-con-boxed e-con e-parent\" data-id=\"e66482a\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-b75d09a elementor-widget elementor-widget-text-editor\" data-id=\"b75d09a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Prodotti Gianni collaborates with various partners to provide researchers with a broad and complete line for flow cytometric analysis.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-28162c6 elementor-widget elementor-widget-table-of-contents\" data-id=\"28162c6\" data-element_type=\"widget\" data-e-type=\"widget\" data-settings=\"{&quot;headings_by_tags&quot;:[&quot;h3&quot;],&quot;exclude_headings_by_selector&quot;:[],&quot;marker_view&quot;:&quot;bullets&quot;,&quot;no_headings_message&quot;:&quot;Non sono state trovate intestazioni in questa pagina.&quot;,&quot;icon&quot;:{&quot;value&quot;:&quot;fas fa-circle&quot;,&quot;library&quot;:&quot;fa-solid&quot;,&quot;rendered_tag&quot;:&quot;&lt;svg class=\\&quot;e-font-icon-svg e-fas-circle\\&quot; viewBox=\\&quot;0 0 512 512\\&quot; xmlns=\\&quot;http:\\\/\\\/www.w3.org\\\/2000\\\/svg\\&quot;&gt;&lt;path d=\\&quot;M256 8C119 8 8 119 8 256s111 248 248 248 248-111 248-248S393 8 256 8z\\&quot;&gt;&lt;\\\/path&gt;&lt;\\\/svg&gt;&quot;},&quot;hierarchical_view&quot;:&quot;yes&quot;,&quot;min_height&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;min_height_laptop&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;min_height_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;min_height_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"table-of-contents.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-toc__header\">\n\t\t\t\t\t\t<h3 class=\"elementor-toc__header-title\">\n\t\t\t\tFlow Cytometry Line\t\t\t<\/h3>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<div id=\"elementor-toc__28162c6\" class=\"elementor-toc__body\">\n\t\t\t<div class=\"elementor-toc__spinner-container\">\n\t\t\t\t<svg class=\"elementor-toc__spinner eicon-animation-spin e-font-icon-svg e-eicon-loading\" aria-hidden=\"true\" viewBox=\"0 0 1000 1000\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M500 975V858C696 858 858 696 858 500S696 142 500 142 142 304 142 500H25C25 237 238 25 500 25S975 237 975 500 763 975 500 975Z\"><\/path><\/svg>\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-48e9ded e-flex e-con-boxed e-con e-parent\" data-id=\"48e9ded\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-fa8b424 elementor-widget elementor-widget-heading\" data-id=\"fa8b424\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Reagents<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2790699 elementor-widget elementor-widget-spacer\" data-id=\"2790699\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-06e300d elementor-widget elementor-widget-text-editor\" data-id=\"06e300d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ul><li>Antibodies conjugated with classic fluorochromes and brilliant new generation dyes.<\/li><li>Isotype controls<\/li><li>Compensation beads<\/li><li>Reagents and kits for cell viability and apoptosis studies: Annexin V, Tunel kit, Mitochondrial Membrane Potential Assay, Caspase Analysis, Live\/Dead Reagents, Reagents for intracellular staining, Sars-CoV2 Reagents<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-f577da5 e-flex e-con-boxed e-con e-parent\" data-id=\"f577da5\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-f85ec4d elementor-widget elementor-widget-heading\" data-id=\"f85ec4d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Conjugation Kits<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d67988c elementor-widget elementor-widget-spacer\" data-id=\"d67988c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7bb479c elementor-widget elementor-widget-text-editor\" data-id=\"7bb479c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Abcam&#8217;s innovative Lightning-Link\u00ae technology enables direct conjugation of antibodies or proteins with HRP, FITC, PE, biotin and other enzymes, fluorophores and small molecules.<\/p><ul><li>Quick and extremely simple conjugation system.<\/li><li>Minimal amounts of starting material required.<\/li><li>Various formats available and customization possibilities<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-98eb20e e-flex e-con-boxed e-con e-parent\" data-id=\"98eb20e\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-d209f45 elementor-widget elementor-widget-heading\" data-id=\"d209f45\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Exosome Analysis<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8ecbef5 elementor-widget elementor-widget-spacer\" data-id=\"8ecbef5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c55806e elementor-widget elementor-widget-text-editor\" data-id=\"c55806e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Reagents and complete kits for simple and precise detection and characterization of Exosomes.<\/p><ul><li>Single antibodies for recognition of specific target molecules.<\/li><li>Panel of specific antibodies for exosome recognition.<\/li><li>Capture and enrichment beads for exosomes (CD9, CD63, CD81, CD274, CD326).<\/li><li>Lyophilized exosome standards to use as controls.<\/li><li>Kits for isolation with capture beads and immunophenotyping of exosomes with sample-specific markers. Simultaneous isolation and immunophenotyping (CD9+CD63 for cell cultures and urine, CD63+CD81 for plasma). <\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-e53899a e-flex e-con-boxed e-con e-parent\" data-id=\"e53899a\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-ffd4c4e elementor-widget elementor-widget-heading\" data-id=\"ffd4c4e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Multiplexing Analysis<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-947a2af elementor-widget elementor-widget-spacer\" data-id=\"947a2af\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2f81d17 elementor-widget elementor-widget-text-editor\" data-id=\"2f81d17\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Quantitative analysis of up to 25 analytes simultaneously using flow cytometry<\/p><ul><li>Cytometric Bead Array technology for high-throughput protein detection using a standard flow cytometer.<\/li><li>Sandwich immunoassay principle, where a target protein is immobilized between a capture antibody locked on magnetic microspheres and a detection antibody conjugated with a fluorophore.<\/li><li>Simultaneous analysis of multiple target proteins using different combinations of microsphere sizes and fluorophores.<\/li><li>Compatible with most standard flow cytometers equipped with blue and red lasers capable of detecting fluorescence in the phycoerythrin (PE) and allophycocyanin (APC) channels, respectively.<\/li><li>Up to 25 different proteins can be analyzed with just 25\u03bcl of sample.<\/li><li>Rapid technique that is more economical than classic ELISA or WB assays.<br\/>Customizable.<\/li><\/ul><p><span style=\"font-weight: 400\">This technology enables the design and creation of assays to measure a variety of analytes including inflammatory mediators, chemokines, immunoglobulin isotypes, intracellular signaling molecules, apoptotic mediators, adhesion molecules, and antibodies.<\/span><\/p><p><strong>Main Applications:<\/strong><\/p><ul><li>Simultaneous analysis of various cytokines (e.g., inflammatory cytokine analysis).<\/li><li>Identification of potential molecular targets for drug development.<\/li><li>Identification of molecular mechanisms of drug action.<\/li><li>Research for new biomarkers.<\/li><li>Identification of various factors involved in disease expression processes.<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-c8485ae e-flex e-con-boxed e-con e-parent\" data-id=\"c8485ae\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-c8933bc elementor-widget elementor-widget-heading\" data-id=\"c8933bc\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">RayPlex\u00ae Line<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d65d3ea elementor-widget elementor-widget-spacer\" data-id=\"d65d3ea\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8e6cc9a elementor-widget elementor-widget-text-editor\" data-id=\"8e6cc9a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>RayBiotech&#8217;s <strong>RayPlex\u00ae<\/strong> line is a microsphere-based (bead-based) multiplex assay platform designed for the simultaneous detection and quantification of numerous protein analytes in a single sample. It uses <strong>flow cytometry<\/strong> technology, combining the specificity of sandwich assays with the high throughput of multiplex systems. <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-df02080 elementor-widget elementor-widget-image\" data-id=\"df02080\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"800\" height=\"163\" src=\"https:\/\/www.prodottigianni.com\/wp-content\/uploads\/2026\/05\/ray-plex-1024x208.png\" class=\"attachment-large size-large wp-image-9011\" alt=\"ray-plex\" srcset=\"https:\/\/www.prodottigianni.com\/wp-content\/uploads\/2026\/05\/ray-plex-1024x208.png 1024w, https:\/\/www.prodottigianni.com\/wp-content\/uploads\/2026\/05\/ray-plex-300x61.png 300w, https:\/\/www.prodottigianni.com\/wp-content\/uploads\/2026\/05\/ray-plex-768x156.png 768w, https:\/\/www.prodottigianni.com\/wp-content\/uploads\/2026\/05\/ray-plex-1536x312.png 1536w, https:\/\/www.prodottigianni.com\/wp-content\/uploads\/2026\/05\/ray-plex.png 1621w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ddf586a elementor-widget elementor-widget-text-editor\" data-id=\"ddf586a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Main Features<\/strong><\/p><ul><li><strong>Multiplex Analysis:<\/strong> Allows simultaneous measurement of up to <strong>25 or more proteins<\/strong> (such as cytokines, chemokines and growth factors) from a minimal sample volume (only <strong>25 \u00b5l<\/strong>).<\/li><li><strong>Universal Compatibility:<\/strong> Requires no dedicated or proprietary instrumentation; kits are compatible with most <strong>standard cytofluorometers<\/strong> equipped with blue (488 nm) and red (633\/640 nm) lasers.<\/li><li><strong>Efficiency:<\/strong> The operating protocol is rapid (about <strong>4 hours<\/strong> total time) and significantly cheaper (5 to 10 times) than performing individual ELISA tests for each target.<\/li><li><strong>Versatility of Targets:<\/strong> Pre-validated panels are available for human, mouse, rat and pig species, as well as the ability to create <strong>custom panels<\/strong> by choosing from more than 200 validated antibody pairs.<\/li><\/ul><p> <\/p><p><strong>How It Works<\/strong><\/p><p>The system is based on populations of microspheres of different sizes or levels of internal fluorescence. Each population is conjugated with a capture antibody specific for a given analyte: <\/p><ol><li><strong>Capture:<\/strong> The beads are incubated with the sample (serum, plasma or cell supernatant).<\/li><li><strong>Detection:<\/strong> A cocktail of biotinalate detection antibodies is added, followed by streptavidin-phycoerythrin (PE).<\/li><li><strong>Readout:<\/strong> The cytofluorometer identifies the &#8220;bead&#8221; (and thus the analyte) based on its intrinsic fluorescent signature and quantifies its concentration based on the PE signal intensity.<\/li><\/ol>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-99241cb elementor-widget elementor-widget-spacer\" data-id=\"99241cb\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-13b01d9 elementor-widget elementor-widget-heading\" data-id=\"13b01d9\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Lightning-Link\u00ae Conjugation Kit<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-45ea252 elementor-widget elementor-widget-spacer\" data-id=\"45ea252\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5acddf9 elementor-widget elementor-widget-text-editor\" data-id=\"5acddf9\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Abcam <\/strong>&#8216;s <strong>Lightning-Link\u00ae<\/strong> conjugation kits represent one of the most innovative and rapid solutions for direct labeling of antibodies, proteins or peptides. This technology eliminates the need for complex post-conjugation purification steps, ensuring total (100 percent) recovery of valuable material. <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6d6eb00 elementor-widget elementor-widget-image\" data-id=\"6d6eb00\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"512\" height=\"512\" src=\"https:\/\/www.prodottigianni.com\/wp-content\/uploads\/2026\/05\/Lightning-Link.webp\" class=\"attachment-large size-large wp-image-9008\" alt=\"Lightning-Link\" srcset=\"https:\/\/www.prodottigianni.com\/wp-content\/uploads\/2026\/05\/Lightning-Link.webp 512w, https:\/\/www.prodottigianni.com\/wp-content\/uploads\/2026\/05\/Lightning-Link-300x300.webp 300w, https:\/\/www.prodottigianni.com\/wp-content\/uploads\/2026\/05\/Lightning-Link-150x150.webp 150w\" sizes=\"(max-width: 512px) 100vw, 512px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-07baa1a elementor-widget elementor-widget-text-editor\" data-id=\"07baa1a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Abcam&#8217;s <strong>Lightning-Link\u00ae<\/strong> kits are particularly popular in <strong>cytofluorimetry (FACS)<\/strong> because they allow researchers to create custom panels quickly, overcoming the limitations of commercial availability of directly conjugated antibodies.<\/p><p><strong>Specific Benefits for Cytofluorimetry<\/strong><\/p><ul><li><strong>Multiplex Panel Expansion:<\/strong> Allow the use of specific antibodies (rare or homemade clones) that are not commercially available already conjugated to the desired fluorophore.<\/li><li><strong>Reduced Background:<\/strong> Direct conjugation eliminates the need for secondary antibodies, drastically reducing nonspecific binding and simplifying the design of the experiment (no need to worry about antibody source species).<\/li><li><strong>Maximum Sample Recovery:<\/strong> Because there are no washing or purification steps, even very small amounts of antibody (up to <strong>10 \u00b5g<\/strong>) can be marked, which is ideal for rare or expensive reagents.<\/li><li><strong>Wide Choice of Fluorophores:<\/strong> The line includes all major dyes used in modern cytofluorometers:<ul><li><strong>Fluorescent proteins:<\/strong> APC, PE, PerCP (ideal for low-expression targets due to high brightness).<\/li><li><strong>Synthetic dyes:<\/strong> Alexa Fluor\u00ae (488, 555, 594, 647, etc.), DyLight\u00ae and tandem dyes.<\/li><\/ul><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-05e51d2 elementor-widget elementor-widget-spacer\" data-id=\"05e51d2\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-df9ed82 elementor-widget elementor-widget-heading\" data-id=\"df9ed82\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Sysmex offer<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8551aec elementor-widget elementor-widget-spacer\" data-id=\"8551aec\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9c66d66 elementor-widget elementor-widget-text-editor\" data-id=\"9c66d66\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p class=\"isselectedend\"><span lang=\"IT\">Flow Cytometry for Rapid Microbiological Quality Control, mycotoxin analysis, and ploidy and genome size testing.<\/span><\/p><p class=\"isselectedend\"><span lang=\"IT\"><b>RAPID MICROBIOLOGICAL QUALITY CONTROL<\/b><\/span><\/p><p class=\"isselectedend\"><span lang=\"IT\">Rapid microbiology solutions allow batch release in minutes or a few hours, rather than days. Unlike traditional methods based on seeding and incubation, flow cytometry allows accurate and reproducible results to be obtained in an extremely short time. <\/span><\/p><p class=\"isselectedend\"><span lang=\"IT\">With a single instrument platform, both microbiological analysis and mycotoxin testing can be performed, ensuring maximum efficiency with minimal infrastructure.<\/span><\/p><p class=\"isselectedend\"><span lang=\"IT\">Comprehensive service of application consulting, instrumentation, dedicated reagents, implementation support, validation, technical assistance and maintenance.<\/span><\/p><p class=\"isselectedend\"><span lang=\"IT\"><b>FOOD, FEED &#038; SEED ANALYSIS<\/b><\/span><\/p><p class=\"isselectedend\"><span lang=\"IT\">Microbiological and mycotoxin contamination pose significant risks to human and animal health.<\/span><\/p><p class=\"isselectedend\"><span lang=\"IT\">Mycotoxin Test Kits enable the analysis of raw materials, incoming goods and finished products in about 90 minutes, with significantly lower costs than conventional methods such as HPLC or ELISA.<\/span><\/p><p class=\"isselectedend\"><span lang=\"IT\">In addition, CyStain\u2122 BacCount allows targeted monitoring of probiotics and postbiotics during production, providing crucial information on product stability and efficacy.<\/span><\/p><p class=\"isselectedend\"><span lang=\"IT\"><b>MICROBIAL WATER ANALYSIS<\/b><\/span><\/p><p class=\"isselectedend\"><span lang=\"IT\">Safe water is an essential requirement in the food, beverage, cosmetics and many other manufacturing environments.<\/span><\/p><p class=\"isselectedend\"><span lang=\"IT\">With CyStain\u2122 BacCount Total and CyStain\u2122 BacCount Viable, total bacterial load and viable cells can be determined in as little as 20 minutes, directly in the laboratory or even online at production lines. The system is suitable for different types of process water. <\/span><\/p><p class=\"isselectedend\"><span lang=\"IT\"><b>BEVERAGE ANALYSIS<\/b><\/span><\/p><p class=\"isselectedend\"><span lang=\"IT\">The CyStain\u2122 VitalCount Kit enables producers of juices, soft drinks, and alcoholic and non-alcoholic beverages to release products quickly, quantifying live microorganisms within minutes and significantly reducing storage costs.<\/span><\/p><p class=\"isselectedend\"><span lang=\"IT\">For the brewing industry, solutions along the entire production line:<br\/>&#8211; control of mycotoxins on raw materials with SAFIA tests<br\/>&#8211; assessment of yeast quality and viability with YeastControl Viability<br\/>&#8211; monitoring of undesirable microorganisms<br\/>&#8211; control of finished product<\/span><\/p><p class=\"isselectedend\"><span lang=\"IT\">CyStain\u2122 BacCount also allows the effectiveness of Cleaning in Place (CIP) procedures to be verified with rapid, accurate and reproducible analysis directly in the plant.<\/span><\/p><p class=\"isselectedend\"><span lang=\"IT\">For winemakers, quick tools to monitor microbiological status throughout the winemaking process: alcoholic fermentation control with OenoYeast reagents, malolactic fermentation monitoring with CyStain\u2122 GramCount Kit, detection of altering microorganisms such as Dekkera and Brettanomyces, and final control at bottling with CyStain\u2122 OenoCount.<\/span><\/p><p class=\"isselectedend\"><span lang=\"IT\"><b>MICROBIAL COSMETICS ANALYSIS<\/b><\/span><\/p><p class=\"isselectedend\"><span lang=\"IT\">In the cosmetics and personal care industry, microbiological control is essential to ensure consumer safety.<\/span><\/p><p class=\"isselectedend\"><span lang=\"IT\">The CyStain\u2122 VitalCount Cosmetics Kit enables rapid identification of contaminated batches, reducing quarantine time and minimizing the risk of costly recalls. CyStain\u2122 BacCount reagents can be used to monitor process water and verify the effectiveness of cleaning procedures, supporting faster and safer production release. <\/span><\/p><p class=\"isselectedend\"><span lang=\"IT\"><b>PLOIDY &#038; GENOME SIZE ANALYSIS<\/b><\/span><\/p><p class=\"isselectedend\"><span lang=\"IT\">With more than 50 years of experience in DNA content determination by flow cytometry, we offer comprehensive solutions for ploidy and genome size analysis.<\/span><\/p><p class=\"isselectedend\"><span lang=\"IT\">From high-volume sample screening to highly precise analysis of selected plant material, these systems ensure reliable and reproducible results for industrial applications and scientific research.<\/span><\/p><p class=\"isselectedend\"><span lang=\"IT\"><b>EFFICIENT SAMPLE PREPARATION<\/b><\/span><\/p><p class=\"isselectedend\"><span lang=\"IT\">CellTrics\u2122 cell filters enable rapid and cost-effective cell separation. Available in mesh sizes from 5 to 150 \u00b5m, they integrate easily into any sample preparation workflow. Tissues, fluids, plant material or biopsies can be gently filtered without pressure exposure, preserving cellular integrity and function.  <\/span><\/p><p class=\"isselectedend\"><span lang=\"IT\"><b>ACCELERATE QUALITY CONTROL PROCESSES<\/b><\/span><\/p><p class=\"isselectedend\"><span lang=\"IT\">Reduced waiting time, increased process safety and optimized operating costs-these are the hallmarks of these application solutions.<\/span><\/p><p class=\"isselectedend\"><span lang=\"IT\">Gianni Products&#8217; Life Science department supports scientific research with specialized products and solutions for genomics, transcriptomics, molecular biology and beyond. With qualified international suppliers, technical and scientific expertise and a consultative approach, it supports researchers and laboratories by offering high-quality products, application support and dedicated know-how. <\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Flow Cytometry Line A broad and complete line for flow cytometric analysis. Prodotti Gianni collaborates with various partners to provide researchers with a broad and complete line for flow cytometric analysis. Flow Cytometry Line Reagents Antibodies conjugated with classic fluorochromes and brilliant new generation dyes. Isotype controls Compensation beads Reagents and kits for cell viability [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"_mc_calendar":[],"footnotes":""},"class_list":["post-6656","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\r\n<title>Flow Cytometry - Prodotti Gianni<\/title>\r\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\r\n<link rel=\"canonical\" href=\"https:\/\/www.prodottigianni.com\/en\/life-science-services\/flow-cytometry\/\" \/>\r\n<meta property=\"og:locale\" content=\"en_US\" \/>\r\n<meta property=\"og:type\" content=\"article\" \/>\r\n<meta property=\"og:title\" content=\"Flow Cytometry - Prodotti Gianni\" \/>\r\n<meta property=\"og:description\" content=\"Flow Cytometry Line A broad and complete line for flow cytometric analysis. Prodotti Gianni collaborates with various partners to provide researchers with a broad and complete line for flow cytometric analysis. Flow Cytometry Line Reagents Antibodies conjugated with classic fluorochromes and brilliant new generation dyes. 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Prodotti Gianni collaborates with various partners to provide researchers with a broad and complete line for flow cytometric analysis. Flow Cytometry Line Reagents Antibodies conjugated with classic fluorochromes and brilliant new generation dyes. 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