{"id":6687,"date":"2024-11-15T12:52:05","date_gmt":"2024-11-15T12:52:05","guid":{"rendered":"https:\/\/staging.prodottigianni.com\/service\/"},"modified":"2026-09-07T09:24:20","modified_gmt":"2026-09-07T09:24:20","slug":"service","status":"publish","type":"page","link":"https:\/\/www.prodottigianni.com\/en\/life-science-services\/service\/","title":{"rendered":"Service"},"content":{"rendered":"\t\t
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Gianni Products Service<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Prodotti Gianni provides the \u201cextensive experience of its partners for the\u201d reliable execution of service experiments and custom production requests.<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Next-Generation Sequencing<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Every type of NGS sequencing<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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  • Whole-genome sequencing:<\/strong> identification of SNPs, CNVs, PAVs, insertions, deletions, and translocations<\/li>
  • Bisulfite treatment:<\/strong> assessment of DNA methylation levels<\/li>
  • Whole-exome sequencing:<\/strong> sequencing of the coding region only, with the option to select exons of interest<\/li>
  • Amplicon sequencing:<\/strong> clinical genotyping projects for the analysis of numerous samples at reduced costs<\/li>
  • Long-read sequencing:<\/strong> for the reconstruction of complex genomes<\/li>
  • Metagenomics:<\/strong> Profiling the Taxonomic Composition of Complex Biological Substrates<\/li>
  • Hi-C Seq:<\/strong> a study of chromatin interactions in 3D space by linking chromosome structure directly to the genomic sequence.<\/li>
  • Total RNA-seq:<\/strong> Quantification of Total RNA<\/li>
  • mRNA-seq:<\/strong> quantification through whole-transcript sequencing<\/li>
  • Small RNA-seq:<\/strong> quantification of small RNAs only. Option to select only physiologically relevant small RNAs <\/li>
  • QuantSeq:<\/strong> Differential Expression Analysis. The gold standard for particularly challenging samples, such as FFPE or degraded samples <\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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    • HD Single-Cell RNA-Seq<\/strong>Up to 15,000 genes. RNA quantification from a SINGLE nucleus or a SINGLE cytoplasm using a patented direct RNA amplification approach (minimum RNA input: 0.15 pg) <\/li>
    • 3D Genomics<\/strong>Analysis of the three-dimensional organization of DNA in the nucleus to better understand gene regulation, cell development, disease mechanisms and progression, biomarker discovery, and genome assembly<\/li>
    • Spatial transcriptomics<\/strong>Single-cell transcriptomic analysis of histological samples using the NGS Stereo-Seq approach.<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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      Molecular Biology:<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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      • Gene synthesis (up to 40,000 bases) with \u201ccodon optimization\u201d<\/li>
      • Oligo and Peptide Synthesis<\/li>
      • Cloning and Subcloning<\/li>
      • Mutagenesis<\/li>
      • RNA Synthesis<\/li>
      • DNA library synthesis (site-directed mutagenesis library, random mutant library, degenerate library, controlled library, sgRNA library)<\/li>
      • CRISPR\/Cas9<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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        Multiplex Protein Analysis<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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        Luminex Technology<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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        • Up to 45 cytokines quantified simultaneously in 25 \u03bcL of serum, plasma, or cell supernatant<\/li>
        • Negative control consisting of cytokine-depleted serum for accurate quantification of cytokines<\/li>
        • Significant reduction in costs and turnaround time compared to the equivalent ELISA approach<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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          Array Technology<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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          • Simultaneous quantification of more than 1,000 proteins in serum, plasma, and cell supernatant samples<\/li>
          • Quantification of Glycosylation and Phosphorylation Levels<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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            Custom Development of Proteins, Antibodies, and ELISA Assays<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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            Expression, purification, and characterization of proteins and antibodies including:<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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            • cDNA Cloning<\/li>
            • Expression in 5 cell lines (E. coli, yeast, insect cells, CHO, HEK293)<\/li>
            • Various tags available (HA, GST, His, hFc, AVI, no tag)<\/li>
            • Bulk production capability<\/li>
            • Monoclonal and polyclonal antibodies<\/li>
            • Bifunctional antibodies<\/li>
            • Humanized antibodies<\/li>
            • Nanobodies (antibodies with a molecular weight of 12\u201315 kDa)<\/li>
            • Conjugation of Antibodies\/Nanobodies<\/li>
            • Coating Beads with Antibodies\/Proteins<\/li>
            • ELISA kit development: antibody labeling, antibody pair screening, assay development and optimization, production<\/li>
            • Production of proteins\/antibodies using a patented \u201ccell-free\u201d system<\/li>
            • Production of transmembrane proteins<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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              Analysis of Extracellular Vesicles<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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              • Isolation and purification from any type of sample. Guaranteed optimal purity and recovery, compatible with any downstream processing or analysis <\/li>
              • Characterization using various techniques (TAM, WB, NTA, FC, ELISA)<\/li>
              • Antibody conjugation, nanobodies, agonist molecules, cytokines, anti-phagocytic molecules, reporters, fluorescent probes<\/li>
              • Loading of therapeutic agents, RNA, DNA, peptides, ASOs, and plasmids<\/li>
              • Transcriptome, proteome, and phosphoproteome analysis using state-of-the-art mass spectrometry techniques<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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                Custom Chemistry Services<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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                • Custom manufacturing of APIs, manufacturing intermediates, building blocks, and biochemical compounds<\/li>
                • Preparation of libraries by selecting from > 20,000 chemical compounds of various types (inhibitors, activators, natural products, peptides, antibodies) and specifying the quantity, format (solid or in DMSO), placement in the plates, and concentration.<\/li>
                • CADD (computer-aided drug design) and virtual screening for the computational identification of structures most likely to bind a molecular target of interest.<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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                  Mass Spectrometry Services<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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                  • Accurate Molecular Weight of Peptides and Proteins<\/li>
                  • Peptide mapping, post-translational modifications, and variants<\/li>
                  • LC-MS Analysis of Proteins<\/li>
                  • Peptide Sequencing by Tandem Mass Spectrometry<\/li>
                  • Glycoprotein Analysis<\/li>
                  • Analysis of oligonucleotides, oligosaccharides, and small molecules in general<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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                    Proteomics Services<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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                    • Separation of Protein Samples by SDS-PAGE<\/li>
                    • Protein Identification by LC-MS\/MS<\/li>
                    • Differential proteomics experiments (untargeted, label-free) combined with analysis<\/li>
                    • LC-MS\/MS for protein identification and relative quantification (including statistical analysis)<\/li>
                    • “Targeted” analysis for relative and\/or absolute protein quantification using MRM<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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                      Interactomics Services<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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                      • Analysis of interactions between biomolecules or between biomolecules and generic ligands using Surface Plasmon Resonance<\/li>
                      • Protein Purification Using Affinity Chromatography and\/or Immunoprecipitation (Interattomica)<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

                        Gianni Products Service Prodotti Gianni provides the \u201cextensive experience of its partners for the\u201d reliable execution of service experiments and custom production requests. Next-Generation Sequencing Every type of NGS sequencing Whole-genome sequencing: identification of SNPs, CNVs, PAVs, insertions, deletions, and translocations Bisulfite treatment: assessment of DNA methylation levels Whole-exome sequencing: sequencing of the coding region […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"_mc_calendar":[],"footnotes":"","_members_access_role":[],"_members_access_error":""},"class_list":["post-6687","page","type-page","status-publish","hentry"],"yoast_head":"\r\nService - Prodotti Gianni<\/title>\r\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\r\n<link rel=\"canonical\" href=\"https:\/\/www.prodottigianni.com\/en\/life-science-services\/service\/\" \/>\r\n<meta property=\"og:locale\" content=\"en_US\" \/>\r\n<meta property=\"og:type\" content=\"article\" \/>\r\n<meta property=\"og:title\" content=\"Service - Prodotti Gianni\" \/>\r\n<meta property=\"og:description\" content=\"Gianni Products Service Prodotti Gianni provides the \u201cextensive experience of its partners for the\u201d reliable execution of service experiments and custom production requests. Next-Generation Sequencing Every type of NGS sequencing Whole-genome sequencing: identification of SNPs, CNVs, PAVs, insertions, deletions, and translocations Bisulfite treatment: assessment of DNA methylation levels Whole-exome sequencing: sequencing of the coding region […]\" \/>\r\n<meta property=\"og:url\" content=\"https:\/\/www.prodottigianni.com\/en\/life-science-services\/service\/\" \/>\r\n<meta property=\"og:site_name\" content=\"Prodotti Gianni\" \/>\r\n<meta property=\"article:modified_time\" content=\"2026-09-07T09:24:20+00:00\" \/>\r\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\r\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"3 minutes\" \/>\r\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.prodottigianni.com\\\/en\\\/life-science-services\\\/service\\\/\",\"url\":\"https:\\\/\\\/www.prodottigianni.com\\\/en\\\/life-science-services\\\/service\\\/\",\"name\":\"Service - Prodotti Gianni\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.prodottigianni.com\\\/en\\\/#website\"},\"datePublished\":\"2024-11-15T12:52:05+00:00\",\"dateModified\":\"2026-09-07T09:24:20+00:00\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/www.prodottigianni.com\\\/en\\\/life-science-services\\\/service\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/www.prodottigianni.com\\\/en\\\/life-science-services\\\/service\\\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/www.prodottigianni.com\\\/en\\\/life-science-services\\\/service\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/www.prodottigianni.com\\\/en\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Service\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/www.prodottigianni.com\\\/en\\\/#website\",\"url\":\"https:\\\/\\\/www.prodottigianni.com\\\/en\\\/\",\"name\":\"Prodotti Gianni\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/www.prodottigianni.com\\\/en\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\r\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Service - Prodotti Gianni","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.prodottigianni.com\/en\/life-science-services\/service\/","og_locale":"en_US","og_type":"article","og_title":"Service - Prodotti Gianni","og_description":"Gianni Products Service Prodotti Gianni provides the \u201cextensive experience of its partners for the\u201d reliable execution of service experiments and custom production requests. Next-Generation Sequencing Every type of NGS sequencing Whole-genome sequencing: identification of SNPs, CNVs, PAVs, insertions, deletions, and translocations Bisulfite treatment: assessment of DNA methylation levels Whole-exome sequencing: sequencing of the coding region […]","og_url":"https:\/\/www.prodottigianni.com\/en\/life-science-services\/service\/","og_site_name":"Prodotti Gianni","article_modified_time":"2026-09-07T09:24:20+00:00","twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"3 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.prodottigianni.com\/en\/life-science-services\/service\/","url":"https:\/\/www.prodottigianni.com\/en\/life-science-services\/service\/","name":"Service - Prodotti Gianni","isPartOf":{"@id":"https:\/\/www.prodottigianni.com\/en\/#website"},"datePublished":"2024-11-15T12:52:05+00:00","dateModified":"2026-09-07T09:24:20+00:00","breadcrumb":{"@id":"https:\/\/www.prodottigianni.com\/en\/life-science-services\/service\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.prodottigianni.com\/en\/life-science-services\/service\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/www.prodottigianni.com\/en\/life-science-services\/service\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.prodottigianni.com\/en\/"},{"@type":"ListItem","position":2,"name":"Service"}]},{"@type":"WebSite","@id":"https:\/\/www.prodottigianni.com\/en\/#website","url":"https:\/\/www.prodottigianni.com\/en\/","name":"Prodotti Gianni","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.prodottigianni.com\/en\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"}]}},"permalink_manager":null,"_links":{"self":[{"href":"https:\/\/www.prodottigianni.com\/en\/wp-json\/wp\/v2\/pages\/6687","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.prodottigianni.com\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.prodottigianni.com\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.prodottigianni.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.prodottigianni.com\/en\/wp-json\/wp\/v2\/comments?post=6687"}],"version-history":[{"count":5,"href":"https:\/\/www.prodottigianni.com\/en\/wp-json\/wp\/v2\/pages\/6687\/revisions"}],"predecessor-version":[{"id":9841,"href":"https:\/\/www.prodottigianni.com\/en\/wp-json\/wp\/v2\/pages\/6687\/revisions\/9841"}],"wp:attachment":[{"href":"https:\/\/www.prodottigianni.com\/en\/wp-json\/wp\/v2\/media?parent=6687"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}