{"id":6687,"date":"2024-11-15T12:52:05","date_gmt":"2024-11-15T12:52:05","guid":{"rendered":"https:\/\/staging.prodottigianni.com\/service\/"},"modified":"2025-10-02T11:23:57","modified_gmt":"2025-10-02T11:23:57","slug":"service","status":"publish","type":"page","link":"https:\/\/www.prodottigianni.com\/en\/life-science-services\/service\/","title":{"rendered":"Service"},"content":{"rendered":"\t\t
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Gianni Products Service<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Prodotti Gianni provides the \u201cextensive experience of its partners for the\u201d reliable execution of service experiments and custom production requests.<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Next Generation Sequencing<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Every type of NGS sequencing<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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  • Whole genome sequencing:<\/strong> identification of SNPs, CNVs, PAVs, insertions, deletions, translocations<\/li>
  • Bisulfite treatment:<\/strong> evaluation of DNA methylation levels<\/li>
  • Whole exome sequencing:<\/strong> sequencing of only the coding part with the possibility of selecting exons of interest<\/li>
  • Amplicon sequencing:<\/strong> clinical genotyping projects for analyzing numerous samples at reduced costs<\/li>
  • Long reads sequencing:<\/strong> for the reconstruction of complex genomes<\/li>
  • Metagenomics:<\/strong> profiling of the taxonomic composition of complex biological substrates<\/li>
  • Hi-C Seq:<\/strong> study of chromatin interactions in 3D space by linking chromosomal structure directly to genomic sequence.<\/li>
  • Total RNAseq:<\/strong> quantification of total RNA<\/li>
  • mRNAseq:<\/strong> quantification through complete sequencing of all messenger RNAs<\/li>
  • Small RNAseq:<\/strong> quantification of Small RNAs only. Possibility to select only physiologically relevant Small RNAs <\/li>
  • QuantSeq:<\/strong> differential expression analysis. Gold standard for particularly challenging samples such as FFPE or degraded samples <\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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    • SINGLE Cell RNAseq in HD version<\/strong>
      Up to 15,000 genes. Quantification of \u201cRNA from A SINGLE nucleus or A SINGLE CYTOPLASM thanks to the patented approach of\u201d direct \u201camplification of\u201d RNA (minimum RNA input – 0.15pg) <\/li>
    • 3D Genomics<\/strong>
      Analysis of the three-dimensional organization of DNA in the nucleus for a better understanding of gene regulation, cell development, disease mechanisms and progression, biomarker research, and genome assembly<\/li>
    • Spatial transcriptomics<\/strong>
      Single Cell transcriptomic analysis on histological samples using NGS Stereo-Seq approach.<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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      Molecular Biology:<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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      • Gene synthesis (up to 40,000 bases) with \u201ccodon optimization\u201d<\/li>
      • Oligo and peptide synthesis<\/li>
      • Cloning and subcloning<\/li>
      • Mutagenesis<\/li>
      • RNA synthesis<\/li>
      • DNA library synthesis (site-directed mutagenesis library, random mutant library, degenerated library, controlled library, sgRNA library)<\/li>
      • CRISPR\/Cas9<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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        Protein Multiplex Analysis<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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        Luminex Technology<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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        • Up to 45 cytokines quantified simultaneously in 25\u03bcl of serum\/plasma\/cell supernatant<\/li>
        • Negative control consisting of cytokine-depleted serum for exact quantification<\/li>
        • Significant reduction in costs and time compared to the corresponding ELISA approach<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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          Array Technology<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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          • Quantification of over 1,000 proteins simultaneously in serum\/plasma\/cell supernatant samples<\/li>
          • Quantification of glycosylation and phosphorylation levels<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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            Proteins\/Antibodies\/ELISA custom development<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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            Expression, purification, and characterization of proteins and antibodies including:<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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            • cDNA cloning<\/li>
            • Expression in 5 cell lines (E.Coli, yeast, insect cells, CHO, HEK293)<\/li>
            • Availability of different tags (HA, GST, His, hFc, AVI, no tag)<\/li>
            • Possibility of bulk production<\/li>
            • Monoclonal and polyclonal antibodies<\/li>
            • Bifunctional antibodies<\/li>
            • Humanized antibodies<\/li>
            • Nanobodies (antibodies with MW 12-15 KDa)<\/li>
            • Conjugation of antibodies\/nanobodies<\/li>
            • Coating of beads with antibodies\/proteins<\/li>
            • Development of ELISA kits: antibody labeling, antibody pair screening, assay development and optimization, production<\/li>
            • Production of proteins\/antibodies with patented \u201ccell free\u201d system<\/li>
            • Production of transmembrane proteins<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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              Extracellular Vesicles Analysis<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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              • Isolation and purification from any type of sample. Guarantee of optimal purity and recovery, compatible with any downstream processing or analysis <\/li>
              • Characterization with different techniques (TAM, WB, NTA, FC, ELISA)<\/li>
              • Conjugation to antibodies, nanobodies, agonist molecules, cytokines, anti-phagocytic molecules, reporters, fluorescent probes<\/li>
              • Loading with therapeutic agents, RNA, DNA, peptides, ASOs, plasmids<\/li>
              • Analysis of transcriptome, proteome, and phosphoproteome using cutting-edge mass spectrometry techniques<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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                Custom Chemistry Services<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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                • Custom production of APIs, production intermediates, building blocks, biochemical compounds<\/li>
                • Setup of libraries selecting from > 20,000 chemical compounds of different nature (inhibitors, activators, natural products, peptides, antibodies) and choosing quantity, format (solid or in
                  DMSO), plate positioning and concentration.<\/li>
                • CADD (computer-aided drug design) and virtual screening for the computational identification of structures most likely to bind a molecular target of interest.<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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                  Mass Spectrometry Services<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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                  • Accurate Molecular Weight of peptides and proteins<\/li>
                  • Peptide mapping, post-translational modifications and variants<\/li>
                  • LC-MS analysis of proteins<\/li>
                  • Peptide sequencing by Tandem Mass Spectrometry<\/li>
                  • Analysis of glycoproteins<\/li>
                  • Analysis of oligonucleotides, oligosaccharides, small molecules in general<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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                    Proteomics Services<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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                    • Separation of protein samples by SDS-PAGE<\/li>
                    • Protein identification by LC-MS\/MS<\/li>
                    • Differential proteomics experiments (\u201cuntargeted label free\u201d type) associated with analysis<\/li>
                    • LC-MS\/MS for identification and relative quantification of proteins (including statistical analysis)<\/li>
                    • \u201cTargeted\u201d analysis for relative and\/or absolute quantification of proteins by MRM<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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                      Interactomics Services<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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                      • Analysis of interactions between biomolecules or biomolecules and generic ligands through Surface Plasmon Resonance<\/li>
                      • Protein purification through affinity chromatography experiments and\/or immunoprecipitation (Interactomics)<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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                        Artificial Intelligence Service for Life Science<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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                        • Identification of genes related to genes of interest using the free GeneRecommender platform<\/li>
                        • Development of AI algorithms<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

                          Gianni Products Service Prodotti Gianni provides the \u201cextensive experience of its partners for the\u201d reliable execution of service experiments and custom production requests. Next Generation Sequencing Every type of NGS sequencing Whole genome sequencing: identification of SNPs, CNVs, PAVs, insertions, deletions, translocations Bisulfite treatment: evaluation of DNA methylation levels Whole exome sequencing: sequencing of only […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"_mc_calendar":[],"footnotes":""},"class_list":["post-6687","page","type-page","status-publish","hentry"],"yoast_head":"\r\nService - Prodotti Gianni<\/title>\r\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\r\n<link rel=\"canonical\" href=\"https:\/\/www.prodottigianni.com\/en\/life-science-services\/service\/\" \/>\r\n<meta property=\"og:locale\" content=\"en_US\" \/>\r\n<meta property=\"og:type\" content=\"article\" \/>\r\n<meta property=\"og:title\" content=\"Service - Prodotti Gianni\" \/>\r\n<meta property=\"og:description\" content=\"Gianni Products Service Prodotti Gianni provides the \u201cextensive experience of its partners for the\u201d reliable execution of service experiments and custom production requests. 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Next Generation Sequencing Every type of NGS sequencing Whole genome sequencing: identification of SNPs, CNVs, PAVs, insertions, deletions, translocations Bisulfite treatment: evaluation of DNA methylation levels Whole exome sequencing: sequencing of only […]","og_url":"https:\/\/www.prodottigianni.com\/en\/life-science-services\/service\/","og_site_name":"Prodotti Gianni","article_modified_time":"2025-10-02T11:23:57+00:00","twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"3 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.prodottigianni.com\/en\/life-science-services\/service\/","url":"https:\/\/www.prodottigianni.com\/en\/life-science-services\/service\/","name":"Service - Prodotti Gianni","isPartOf":{"@id":"https:\/\/www.prodottigianni.com\/en\/#website"},"datePublished":"2024-11-15T12:52:05+00:00","dateModified":"2025-10-02T11:23:57+00:00","breadcrumb":{"@id":"https:\/\/www.prodottigianni.com\/en\/life-science-services\/service\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.prodottigianni.com\/en\/life-science-services\/service\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/www.prodottigianni.com\/en\/life-science-services\/service\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.prodottigianni.com\/en\/"},{"@type":"ListItem","position":2,"name":"Service"}]},{"@type":"WebSite","@id":"https:\/\/www.prodottigianni.com\/en\/#website","url":"https:\/\/www.prodottigianni.com\/en\/","name":"Prodotti Gianni","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.prodottigianni.com\/en\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"}]}},"_links":{"self":[{"href":"https:\/\/www.prodottigianni.com\/en\/wp-json\/wp\/v2\/pages\/6687","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.prodottigianni.com\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.prodottigianni.com\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.prodottigianni.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.prodottigianni.com\/en\/wp-json\/wp\/v2\/comments?post=6687"}],"version-history":[{"count":4,"href":"https:\/\/www.prodottigianni.com\/en\/wp-json\/wp\/v2\/pages\/6687\/revisions"}],"predecessor-version":[{"id":7496,"href":"https:\/\/www.prodottigianni.com\/en\/wp-json\/wp\/v2\/pages\/6687\/revisions\/7496"}],"wp:attachment":[{"href":"https:\/\/www.prodottigianni.com\/en\/wp-json\/wp\/v2\/media?parent=6687"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}