Gianni Products Service

Prodotti Gianni provides the “extensive experience of its partners for the” reliable execution of service experiments and custom production requests.

Next-Generation Sequencing

Whole-genome sequencing: identification of SNPs, CNVs, PAVs, insertions, deletions, and translocations
Bisulfite treatment: assessment of DNA methylation levels
Whole-exome sequencing: sequencing of the coding region only, with the option to select exons of interest
Amplicon sequencing: clinical genotyping projects for the analysis of numerous samples at reduced costs
Long-read sequencing: for the reconstruction of complex genomes
Metagenomics: Profiling the Taxonomic Composition of Complex Biological Substrates
Hi-C Seq: a study of chromatin interactions in 3D space by linking chromosome structure directly to the genomic sequence.
Total RNA-seq: Quantification of Total RNA
mRNA-seq: quantification through whole-transcript sequencing
Small RNA-seq: quantification of small RNAs only. Option to select only physiologically relevant small RNAs
QuantSeq: Differential Expression Analysis. The gold standard for particularly challenging samples, such as FFPE or degraded samples

HD Single-Cell RNA-Seq
Up to 15,000 genes. RNA quantification from a SINGLE nucleus or a SINGLE cytoplasm using a patented direct RNA amplification approach (minimum RNA input: 0.15 pg)
3D Genomics
Analysis of the three-dimensional organization of DNA in the nucleus to better understand gene regulation, cell development, disease mechanisms and progression, biomarker discovery, and genome assembly
Spatial transcriptomics
Single-cell transcriptomic analysis of histological samples using the NGS Stereo-Seq approach.

Gene synthesis (up to 40,000 bases) with “codon optimization”
Oligo and Peptide Synthesis
Cloning and Subcloning
Mutagenesis
RNA Synthesis
DNA library synthesis (site-directed mutagenesis library, random mutant library, degenerate library, controlled library, sgRNA library)
CRISPR/Cas9

Up to 45 cytokines quantified simultaneously in 25 μL of serum, plasma, or cell supernatant
Negative control consisting of cytokine-depleted serum for accurate quantification of cytokines
Significant reduction in costs and turnaround time compared to the equivalent ELISA approach

Simultaneous quantification of more than 1,000 proteins in serum, plasma, and cell supernatant samples
Quantification of Glycosylation and Phosphorylation Levels

cDNA Cloning
Expression in 5 cell lines (E. coli, yeast, insect cells, CHO, HEK293)
Various tags available (HA, GST, His, hFc, AVI, no tag)
Bulk production capability
Monoclonal and polyclonal antibodies
Bifunctional antibodies
Humanized antibodies
Nanobodies (antibodies with a molecular weight of 12–15 kDa)
Conjugation of Antibodies/Nanobodies
Coating Beads with Antibodies/Proteins
ELISA kit development: antibody labeling, antibody pair screening, assay development and optimization, production
Production of proteins/antibodies using a patented “cell-free” system
Production of transmembrane proteins

Isolation and purification from any type of sample. Guaranteed optimal purity and recovery, compatible with any downstream processing or analysis
Characterization using various techniques (TAM, WB, NTA, FC, ELISA)
Antibody conjugation, nanobodies, agonist molecules, cytokines, anti-phagocytic molecules, reporters, fluorescent probes
Loading of therapeutic agents, RNA, DNA, peptides, ASOs, and plasmids
Transcriptome, proteome, and phosphoproteome analysis using state-of-the-art mass spectrometry techniques

Custom manufacturing of APIs, manufacturing intermediates, building blocks, and biochemical compounds
Preparation of libraries by selecting from > 20,000 chemical compounds of various types (inhibitors, activators, natural products, peptides, antibodies) and specifying the quantity, format (solid or in DMSO
), placement in the plates, and concentration.
CADD (computer-aided drug design) and virtual screening for the computational identification of structures most likely to bind a molecular target of interest.

Separation of Protein Samples by SDS-PAGE
Protein Identification by LC-MS/MS
Differential proteomics experiments (untargeted, label-free) combined with analysis
LC-MS/MS for protein identification and relative quantification (including statistical analysis)
“Targeted” analysis for relative and/or absolute protein quantification using MRM

Analysis of interactions between biomolecules or between biomolecules and generic ligands using Surface Plasmon Resonance
Protein Purification Using Affinity Chromatography and/or Immunoprecipitation (Interattomica)