Flow Cytometry Line

A broad and complete line for flow cytometric analysis.

Prodotti Gianni collaborates with various partners to provide researchers with a broad and complete line for flow cytometric analysis.

Reagents

Antibodies conjugated with classic fluorochromes and brilliant new generation dyes.
Isotype controls
Compensation beads
Reagents and kits for cell viability and apoptosis studies: Annexin V, Tunel kit, Mitochondrial Membrane Potential Assay, Caspase Analysis, Live/Dead Reagents, Reagents for intracellular staining, Sars-CoV2 Reagents

Conjugation Kits

Abcam’s innovative Lightning-Link® technology enables direct conjugation of antibodies or proteins with HRP, FITC, PE, biotin and other enzymes, fluorophores and small molecules.

Quick and extremely simple conjugation system.
Minimal amounts of starting material required.
Various formats available and customization possibilities

Exosome Analysis

Reagents and complete kits for simple and precise detection and characterization of Exosomes.

Single antibodies for recognition of specific target molecules.
Panel of specific antibodies for exosome recognition.
Capture and enrichment beads for exosomes (CD9, CD63, CD81, CD274, CD326).
Lyophilized exosome standards to use as controls.
Kits for isolation with capture beads and immunophenotyping of exosomes with sample-specific markers. Simultaneous isolation and immunophenotyping (CD9+CD63 for cell cultures and urine, CD63+CD81 for plasma).

Multiplexing Analysis

Quantitative analysis of up to 25 analytes simultaneously using flow cytometry

Cytometric Bead Array technology for high-throughput protein detection using a standard flow cytometer.
Sandwich immunoassay principle, where a target protein is immobilized between a capture antibody locked on magnetic microspheres and a detection antibody conjugated with a fluorophore.
Simultaneous analysis of multiple target proteins using different combinations of microsphere sizes and fluorophores.
Compatible with most standard flow cytometers equipped with blue and red lasers capable of detecting fluorescence in the phycoerythrin (PE) and allophycocyanin (APC) channels, respectively.
Up to 25 different proteins can be analyzed with just 25μl of sample.
Rapid technique that is more economical than classic ELISA or WB assays.
Customizable.

This technology enables the design and creation of assays to measure a variety of analytes including inflammatory mediators, chemokines, immunoglobulin isotypes, intracellular signaling molecules, apoptotic mediators, adhesion molecules, and antibodies.

Main Applications:

Simultaneous analysis of various cytokines (e.g., inflammatory cytokine analysis).
Identification of potential molecular targets for drug development.
Identification of molecular mechanisms of drug action.
Research for new biomarkers.
Identification of various factors involved in disease expression processes.

RayPlex® Line

RayBiotech’s RayPlex® line is a microsphere-based (bead-based) multiplex assay platform designed for the simultaneous detection and quantification of numerous protein analytes in a single sample. It uses flow cytometry technology, combining the specificity of sandwich assays with the high throughput of multiplex systems.

Main Features

Multiplex Analysis: Allows simultaneous measurement of up to 25 or more proteins (such as cytokines, chemokines and growth factors) from a minimal sample volume (only 25 µl).
Universal Compatibility: Requires no dedicated or proprietary instrumentation; kits are compatible with most standard cytofluorometers equipped with blue (488 nm) and red (633/640 nm) lasers.
Efficiency: The operating protocol is rapid (about 4 hours total time) and significantly cheaper (5 to 10 times) than performing individual ELISA tests for each target.
Versatility of Targets: Pre-validated panels are available for human, mouse, rat and pig species, as well as the ability to create custom panels by choosing from more than 200 validated antibody pairs.

How It Works

The system is based on populations of microspheres of different sizes or levels of internal fluorescence. Each population is conjugated with a capture antibody specific for a given analyte:

Capture: The beads are incubated with the sample (serum, plasma or cell supernatant).
Detection: A cocktail of biotinalate detection antibodies is added, followed by streptavidin-phycoerythrin (PE).
Readout: The cytofluorometer identifies the “bead” (and thus the analyte) based on its intrinsic fluorescent signature and quantifies its concentration based on the PE signal intensity.

Lightning-Link® Conjugation Kit

Abcam ‘s Lightning-Link® conjugation kits represent one of the most innovative and rapid solutions for direct labeling of antibodies, proteins or peptides. This technology eliminates the need for complex post-conjugation purification steps, ensuring total (100 percent) recovery of valuable material.

Abcam’s Lightning-Link® kits are particularly popular in cytofluorimetry (FACS) because they allow researchers to create custom panels quickly, overcoming the limitations of commercial availability of directly conjugated antibodies.

Specific Benefits for Cytofluorimetry

Multiplex Panel Expansion: Allow the use of specific antibodies (rare or homemade clones) that are not commercially available already conjugated to the desired fluorophore.
Reduced Background: Direct conjugation eliminates the need for secondary antibodies, drastically reducing nonspecific binding and simplifying the design of the experiment (no need to worry about antibody source species).
Maximum Sample Recovery: Because there are no washing or purification steps, even very small amounts of antibody (up to 10 µg) can be marked, which is ideal for rare or expensive reagents.
Wide Choice of Fluorophores: The line includes all major dyes used in modern cytofluorometers:
- Fluorescent proteins: APC, PE, PerCP (ideal for low-expression targets due to high brightness).
- Synthetic dyes: Alexa Fluor® (488, 555, 594, 647, etc.), DyLight® and tandem dyes.

Sysmex offer

Flow Cytometry for Rapid Microbiological Quality Control, mycotoxin analysis, and ploidy and genome size testing.

RAPID MICROBIOLOGICAL QUALITY CONTROL

Rapid microbiology solutions allow batch release in minutes or a few hours, rather than days. Unlike traditional methods based on seeding and incubation, flow cytometry allows accurate and reproducible results to be obtained in an extremely short time.

With a single instrument platform, both microbiological analysis and mycotoxin testing can be performed, ensuring maximum efficiency with minimal infrastructure.

Comprehensive service of application consulting, instrumentation, dedicated reagents, implementation support, validation, technical assistance and maintenance.

FOOD, FEED & SEED ANALYSIS

Microbiological and mycotoxin contamination pose significant risks to human and animal health.

Mycotoxin Test Kits enable the analysis of raw materials, incoming goods and finished products in about 90 minutes, with significantly lower costs than conventional methods such as HPLC or ELISA.

In addition, CyStain™ BacCount allows targeted monitoring of probiotics and postbiotics during production, providing crucial information on product stability and efficacy.

MICROBIAL WATER ANALYSIS

Safe water is an essential requirement in the food, beverage, cosmetics and many other manufacturing environments.

With CyStain™ BacCount Total and CyStain™ BacCount Viable, total bacterial load and viable cells can be determined in as little as 20 minutes, directly in the laboratory or even online at production lines. The system is suitable for different types of process water.

BEVERAGE ANALYSIS

The CyStain™ VitalCount Kit enables producers of juices, soft drinks, and alcoholic and non-alcoholic beverages to release products quickly, quantifying live microorganisms within minutes and significantly reducing storage costs.

For the brewing industry, solutions along the entire production line:
– control of mycotoxins on raw materials with SAFIA tests
– assessment of yeast quality and viability with YeastControl Viability
– monitoring of undesirable microorganisms
– control of finished product

CyStain™ BacCount also allows the effectiveness of Cleaning in Place (CIP) procedures to be verified with rapid, accurate and reproducible analysis directly in the plant.

For winemakers, quick tools to monitor microbiological status throughout the winemaking process: alcoholic fermentation control with OenoYeast reagents, malolactic fermentation monitoring with CyStain™ GramCount Kit, detection of altering microorganisms such as Dekkera and Brettanomyces, and final control at bottling with CyStain™ OenoCount.

MICROBIAL COSMETICS ANALYSIS

In the cosmetics and personal care industry, microbiological control is essential to ensure consumer safety.

The CyStain™ VitalCount Cosmetics Kit enables rapid identification of contaminated batches, reducing quarantine time and minimizing the risk of costly recalls. CyStain™ BacCount reagents can be used to monitor process water and verify the effectiveness of cleaning procedures, supporting faster and safer production release.

PLOIDY & GENOME SIZE ANALYSIS

With more than 50 years of experience in DNA content determination by flow cytometry, we offer comprehensive solutions for ploidy and genome size analysis.

From high-volume sample screening to highly precise analysis of selected plant material, these systems ensure reliable and reproducible results for industrial applications and scientific research.

EFFICIENT SAMPLE PREPARATION

CellTrics™ cell filters enable rapid and cost-effective cell separation. Available in mesh sizes from 5 to 150 µm, they integrate easily into any sample preparation workflow. Tissues, fluids, plant material or biopsies can be gently filtered without pressure exposure, preserving cellular integrity and function.

ACCELERATE QUALITY CONTROL PROCESSES

Reduced waiting time, increased process safety and optimized operating costs-these are the hallmarks of these application solutions.

Gianni Products’ Life Science department supports scientific research with specialized products and solutions for genomics, transcriptomics, molecular biology and beyond. With qualified international suppliers, technical and scientific expertise and a consultative approach, it supports researchers and laboratories by offering high-quality products, application support and dedicated know-how.